High throughput quantitative PCR facility

Genomic Paris Centre – ENS PSL


The qPCR-HD-GPC platform offers public and private research teams, as well as industry, expertise and methods for high-throughput quantitative genome and transcriptome analysis. The microfluidic technologies implemented on the platform enable all quantitative and digital PCR methods to be used for genomic analyses such as :

    • Gene Expression variation (GE)
    • Copy number variation (CNV)
    • Search for polymorphisms (SNP)
    • Preparation and quantification of libraries for high-throughput sequencing
    • Single cell genomics/trancriptomics
      Absolute quantification (dPCR and cdPCR)


In September 2013, the qPCR-HD-GPC platform was opened to provide high-throughput quantitative PCR services available to the entire scientific community. The platform was then integrated into the Montagne Sainte-Geneviève technology platform network and offered new quantitative genomic analysis capabilities in connection with existing high-throughput sequencing services (ENS and Institut Curie), Laser Microdissection (Collège de France) and Flow Cytometry (ENS and Institut Curie).


The platform has been labelled IBiSA since 2018 (label renewed in 2021) and is part of the Genomic Paris Centre consortium, which includes the ENS Genomics and High Throughput qPCR platforms. Genomic Paris Centre is part of the core of the France Genomics Infrastructure since May 2020. In this sense, our objectives are to :

    • Make quantitative genomic analysis technologies accessible to all laboratories
    • Support research teams in the success of their projects
    • Disseminate large-scale approaches in quantitative and functional genomics within the scientific community
    • Train and inform on the advances in the field

years (since 2013)

related publications



Who, what and how ?

The qPCR-HD-GPC platform is handled by a biologist and a platform engineer. The platform is equipped with the Biomark-HDTM system from Fluidigm for quantitative and digital PCR in microfluidics, and with the NaicaTM system from Stilla Technologies for digital PCR in high-resolution nanodroplets (cdPCR). We offer a service based on a collaboration between the user and the qPCR-HD-GPC facility, that includes experimental design and analysis aimed at delivering publication-ready results. Supported samples include RNA, miRNA, cDNA, genomic DNA and environmental DNA from tissue or single cells. Quantitative and statistics analyses of the results are available with R.

Bertrand DUCOS

Scientific responsible, Ph.D

Research engineer CNRS


Technical responsible,

study engineer PSL

Latest News

Publication: “A genome-wide screen reveals new regulators of the 2-cell-like cell state”

Nature Structural & Molecular Biology (2023). Nikhil Gupta, Lounis Yakhou, Julien Richard Albert, Anaelle Azogui,...

Publication: “Presenilin natural antisense transcription in the sea urchin: a general control of gene expression during development?”

Journal of Cell Science. 2023 Jun 22 ; jcs.261284. doi: 10.1242/jcs.261284. Odile Bronchain, Bertrand Ducos, Harald...

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PNAS May 19, 2022. 119 (21) e2122544119. https://doi.org/10.1073/pnas.2122544119 Maxime Veleanu, Beetsi...

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Equipment upgrade: installation of Prism6 from Stilla Technologies

Installation of Prism6 from Stilla Technologies, for digital PCR analysis in nanodroplets with 6 detection channels....

Publication: “Objective Comparison of High Throughput qPCR Data Analysis Methods”

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Our partners

Département de physique ENS
Région Ile de France
Memo Life Labex
Collège de France
Institut Curie
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