High throughput quantitative PCR facility

Genomic Paris Centre – ENS PSL

Presentation

The qPCR-HD-GPC platform offers public and private research teams, as well as industry, expertise and methods for high-throughput quantitative genome and transcriptome analysis. The microfluidic technologies implemented on the platform enable all quantitative and digital PCR methods to be used for genomic analyses such as :

    • Gene Expression variation (GE)
    • Copy number variation (CNV)
    • Search for polymorphisms (SNP)
      Genotyping
    • Preparation and quantification of libraries for high-throughput sequencing
    • Single cell genomics/trancriptomics
      Absolute quantification (dPCR and cdPCR)

History

In September 2013, the qPCR-HD-GPC platform was opened to provide high-throughput quantitative PCR services available to the entire scientific community. The platform was then integrated into the Montagne Sainte-Geneviève technology platform network and offered new quantitative genomic analysis capabilities in connection with existing high-throughput sequencing services (ENS and Institut Curie), Laser Microdissection (Collège de France) and Flow Cytometry (ENS and Institut Curie).

Labels

The platform has been labelled IBiSA since 2018 (label renewed in 2021) and is part of the Genomic Paris Centre consortium, which includes the ENS Genomics and High Throughput qPCR platforms. Genomic Paris Centre is part of the core of the France Genomics Infrastructure since May 2020. In this sense, our objectives are to :

    • Make quantitative genomic analysis technologies accessible to all laboratories
    • Support research teams in the success of their projects
    • Disseminate large-scale approaches in quantitative and functional genomics within the scientific community
    • Train and inform on the advances in the field

years (since 2013)

related publications

projects

engineers

Who, what and how ?

The qPCR-HD-GPC platform is handled by a biologist and a platform engineer. The platform is equipped with the Biomark-HDTM system from Fluidigm for quantitative and digital PCR in microfluidics, and with the NaicaTM system from Stilla Technologies for digital PCR in high-resolution nanodroplets (cdPCR). We offer a service based on a collaboration between the user and the qPCR-HD-GPC facility, that includes experimental design and analysis aimed at delivering publication-ready results. Supported samples include RNA, miRNA, cDNA, genomic DNA and environmental DNA from tissue or single cells. Quantitative and statistics analyses of the results are available with R.

Bertrand DUCOS

Scientific responsible, Ph.D

Research engineer CNRS

Marine DELAGRANGE

Technical responsible,

study engineer PSL

Latest News

Publication: “A genome-wide screen reveals new regulators of the 2-cell-like cell state”

Nature Structural & Molecular Biology (2023). Nikhil Gupta, Lounis Yakhou, Julien Richard Albert, Anaelle Azogui,...

Publication: “Presenilin natural antisense transcription in the sea urchin: a general control of gene expression during development?”

Journal of Cell Science. 2023 Jun 22 ; jcs.261284. doi: 10.1242/jcs.261284. Odile Bronchain, Bertrand Ducos, Harald...

Publication: “Modified climbing fiber/Purkinje cell synaptic connectivity in the cerebellum of the neonatal phencyclidine model of schizophrenia”

PNAS May 19, 2022. 119 (21) e2122544119. https://doi.org/10.1073/pnas.2122544119 Maxime Veleanu, Beetsi...

Publication: “Fate and biological impact of persistent luminescence nanoparticles after injection in mice: a one-year follow-up”

Nanoscale, October 2022 DOI:10.1039/D2NR03546D Thomas Lécuyer, Johanne Seguin, Alice Balfourier, Marine Delagrange,...
The Development and Application of Opto-Chemical Tools in the Zebrafish Molecules

Publication: “The Development and Application of Opto-Chemical Tools in the Zebrafish Molecules”

Molecules 2022, 27, 6231. https://doi.org/10.3390/molecules27196231 Zhiping Feng, Bertrand Ducos, Pierluigi Scerbo,...

Publication: “The success of the bloom-forming cyanobacteria Planktothrix: Genotypes variability supports variable responses to light and temperature stress”

Harmful Algae, Volume 117, August 2022, 102285. Sandra Kim Tiam, Katia Comte, Caroline Dalle, Marine Delagrange,...

Publication: “Critical slowing down may account for the robustness of development”

Proc Natl Acad Sci U S A. 2022 Jun 28;119(26):e2205630119. doi: 10.1073/pnas.2205630119. Epub 2022 Jun 22. Zhang W,...

Publication: “Fgf8 dynamics and critical slowing down may account for the temperature independence of somitogenesis”

Communications Biology volume 5, Article number: 113 (2022) Télécharger l'articleWeiting Zhang, Pierluigi...

Equipment upgrade: installation of Prism6 from Stilla Technologies

Installation of Prism6 from Stilla Technologies, for digital PCR analysis in nanodroplets with 6 detection channels....

Publication: “Objective Comparison of High Throughput qPCR Data Analysis Methods”

Research Article,  J Appl Bioinformat Computat Biol S Vol: 10 Issue: 4 Mathieu Bahin, Marine Delagrange, Quentin...

Our partners

ENS PSL
CNRS
Département de physique ENS
PSL
Région Ile de France
GIS IBISA
Memo Life Labex
Collège de France
Institut Curie
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